Fig. 2. Effect of unsaturated fatty acids on palmitate induced NFkB proinflammatory signalling and palmitate uptake. L6 myotubes were incubated for 16 h in media containing D-glucose (5 mM) supplemented with or without palmitate (PA, 0.4 mM) or unsaturated fatty acids palmitoleate (PO, C16:1), Oleate (OL, C18:1) or linoleate (LO, C18:2) at 0.4 mM as indicated prior to analysis of (A) cellular IkBα abundance by immunoblotting and (B) IL6 and β-actin mRNA abundance by qPCR. The data represent the combined analyses of four separate experiments. For PA uptake (C), myotubes were incubated in uptake buffer containing 0.4 mM PA and [3H]-PA (1 mCi/ml) palmitate in the absence and presence of 0.4 mM OL for times indicated. Data in panels A and B are from three separate immunoblotting/qPCR experiments, whereas the uptake data is from six separate experimental determinations. Data is presented as mean ± SEM. Asterisks indicate a significant change (P<0.05) to the control condition (lacking any fatty acid additions) or between the indicated bar values.